General Cell Based Assays
Cayman Chemical HDAC CelBased ActIvIty96
Cayman’s HDAC Cell-Based Assay Kit provides a robust and EAsy-to-use platform for studying HDAC activity modulators in whole cells. By using a cell-permeable HDAC substrate, the activity of various lysine-specific protein deacetylases can be measured in intact cells in a simple and homogenous manner. The fluorescence of the deacetylated reaction product can be analyzed using a fluorometer with an excitation wavelength of 340-360 nm and an emission wavelength of 440-460 nm. An HDAC inhibitor, trichostatin A, is included for checking specificity of the HDAC reaction. This assay parallels Cayman’s HDAC Activity Assay Kit (Item No. 10011563), which uses a nuclear extract rather than whole cells for the assay. Together, both assays will help identify potential inhibitors/activators of HDAC enzymes.
Cayman Chemical NonradIoactIve IodID asy K 96
A B2-AR agonist selectively binds to B1- over B2-ARs (Kds 2.9 and 113 nM respectively) as well as B3-adrenergic B2 bradykinin NK1 and NK2 receptors up to 3 M induces cAMP accumulation in cultured human bronchial epithelial cells relaxes guinea pig tracheal strips precontracted with carbamoylcholine ovalbumin or histamine (pD2s 8.4 9.5 and 9.5 respectively) reverses histamine- and ovalbumin-induced bronchoconstriction in guinea pigs (ED50s 1 and 40 nmol/kg respectively)
Cayman Chemical 12-Lipoxygenase leukocyte-ty
Immunogen: Full length recombinant mouse 12-lipoxygenase (leukocyte-type) • Host: Rabbit • Species Reactivity: (+) Mouse, porcine • Applications: ELISA, WB • MW = ~73 kDa
Abcam Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric)
Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
ASSAYQUANT TECHNOLOGIES INC Protein 1000 kin assay AQT0872
PhosphoSens-Kinetic Kinase Activity Kit 1000 assays - AQT0872
Revvity Health Sciences Inc AlphaScreen HA Detection Kit, 500 Assay Points
AlphaScreen HA Detection Kit, 500 Assay Points
Bioassay Systems QuantiChrom™ Detergent Assay Kit. For quantitative determination of detergents in e.g. water and soil samples.
For quantitative determination of detergents in e.g. water and soil samples. Key Features: Sensitive and accurate. Use 20 µL sample. Linear detection range: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM. Simple and convenient. The procedure involves addition of two reagents and measuring OD560nm or OD650nm. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay. Method: 560nm and 650nm. Samples: Biological and environmental samples. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM.